Case Study: Flow cytometry-based binding and blocking assays for GPC3/CD47 BsAb [92]: To develop a potential immune-modulating therapeutic to treat hepatocellular carcinoma (HCC), the authors designed a novel BsAb directed against the HCC-associated antigen Glypican-3 (GPC3) and CD47, an inhibitory innate immune checkpoint that inhibits ADCP by binding to SIRPa on myeloid cells. The gene discussed is GPC3; the disease is hepatocellular carcinoma.