To examine the role of CXCR4 in response to either docetaxel treatment in the presence of activated macrophages, we knocked down CXCR4 expression in prostate cancer cells including PC-3 and C4-2, with two distinct pairs of short hairpin RNA targeting two different loci in the third exon of human CXCR4, which is shared across five isoforms from the PubMed gene database. This evidence concerns the gene CXCR4 and prostate carcinoma.