PBMCs from healthy, HLA-A2+, melanoma-naive donors were cultured with Flt3 ligand, a standard cocktail of cytokines, and the ELA peptide, either alone or conjugated via its N- (M1) or C-terminus (M2) to PWT2 (Figure 1 and Appendix A). The gene discussed is FLT3LG; the disease is melanoma.