CDH1 and cholangiocarcinoma: Although expression of PD-L1 alone was not significantly associated with poor prognosis in the CCA patient dataset, coordinate downregulation of E-Cad (CDH1) expression and upregulation of VIM expression revealed overall worse survival (HR: 3.09, CI: 1.14~8.41, log-rank equal curves p = 0.020) in combination with PD-L1 (Figure 9A).