In agreement with our findings, a recent study described that disrupting a cysteine protease located in the vacuolar compartment (VAC) of Toxoplasma gondii caused a reduction in the proteolytic activity of parasite lysosomes, the accumulation of undigested autophagosomes in parasite cytoplasm, and subsequently, a reduction in the intensity of infection [53]. This evidence concerns the gene CTSB and infection.