To assess the distinct migratory outcomes of lamin A/C downregulation in B16F10 melanoma cells in vivo, we introduced an experimental lung metastasis model based on intravenous (IV) injection of minute numbers of fluorescently labeled cells (Figure S3), in order to minimize nonphysiological inflammatory responses associated with a bolus of cancer cells that simultaneously enter the lung vasculature. Here, LMNA is linked to melanoma.