Among the hotspot mutations from Rheinbay et al., NBDriver correctly identified mutations in the codon 1047, which are some of the most frequent alterations in the PIK3CA gene [64], mutations in the codon Q61, which is a predominantly mutated hotspot in NRAS implicated in melanoma [62], and evolutionarily conserved residue R132 in IDH1, which was found to be mutated in GBM [65]. Here, NRAS is linked to melanoma.