A previously uncharacterized FGFR3 mutation, Y649C, located in the kinase domain, was found in the MGH156-1A cell line derived from a NSCLC patient who progressed on the first-generation, competitive EGFR TKI erlotinib, followed by a combination of afatinib with a monoclonal antibody (mAb) against EGFR, cetuximab. Here, FGFR3 is linked to non-small cell lung carcinoma.