Methylation frequency: HCC: CDH1 31%, DNMT3b 41%, ESR1 31%CLD: <10% for all 3 genesHealthy subjects: 0%HCC vs. CLDMethylation of any gene (AUC): 0.75; AFP (AUC): 0.63 HCC vs. LCMethylation of any gene (AUC): 0.73; AFP (AUC): 0.62 This evidence concerns the gene ESR1 and hepatocellular carcinoma.