TGR5 methylation frequency: HCC 48%, CLD 14% and healthy subjects 4%HCC vs. CLD (sensitivity/specificity)TGR5 alone: 48.1%/86.4%TGR5 methylation + AFP (200 ng/mL): 68.1%/78.4%AFP (200 ng/mL): 30.6%/92.1% Here, GPBAR1 is linked to congenital secretory chloride diarrhea 1.