TET2 and acute myeloid leukemia: In all cases, the concomitant detection of passenger mutations by NGS is recommended (blue box) for a more accurate prognosis, for example, to predict transformation to MF or AML, as well as to detect mutations associated with increased thrombotic risk, such as TET2. 1The standardized technique according to the European LeukemiaNet (ELN) is based on allele-specific PCR, which allows for the detection of the JAK2V617F mutation as well as the quantification of the allelic frequency [21].