We further demonstrated that an oncolytic adenovirus combined with a prodrug is a powerful tool in cancer therapy, with the ability to convert CB1954 into potentially cytotoxic metabolites by reacting with the NTR enzyme of CRAdNTR, causing tumor lysis and reduction of colorectal cancer growth, while side effects are minimized by avoiding toxicity for vital organs. The gene discussed is NTSR1; the disease is colorectal cancer.