Through behavioral, molecular biology, and electrophysiological experiments, the analgesic effect of N58A on trigeminal neuralgia, the effect of IoN-CCI surgery on Nav1.8 and Nav1.9 in rats’ TG cells, and the protein phosphorylation level of the MAPK pathway all of these things were investigated, in order to provide a theoretical basis for further elucidating the analgesic effect of N58A and its possible mechanism. This evidence concerns the gene SCN11A and trigeminal neuralgia.