The results show that the expressions of both CCDC25 and KLK11 were significantly higher in cancerous tissues than in the adjacent tissues of CCA with lymph node metastasis (p < 0.001 and p = 0.018, respectively) (Figure 3A,C), but not in the CCA without lymph node metastasis (p = 0.057 and p = 0.057, respectively) (Figure 3B,D). The gene discussed is KLK11; the disease is cholangiocarcinoma.