In the search for other possible targets of MARCH8 regulating cell survival, we analyzed the total protein levels and activation by phosphorylation of multiple signaling transducers known to regulate cell survival, such as AKT, ERK, and STAT3, in MDA-MB-231 breast cancer cells that stably express MARCH8-GFP and control GFP. This evidence concerns the gene AKT1 and breast cancer.