Meanwhile, CCK8 analysis showed that cell proliferation was decreased (Figure 4B) and Annexin V/7-AAD staining showed that apoptosis was increased (Figure 4C) in the siCDK1 group compared with the NC group, suggesting that CDK1 knockdown can hinder DNMT3A mutation-related AML progression. This evidence concerns the gene DNMT3A and acute myeloid leukemia.