ASS1 and lung adenocarcinoma: In another study, it was observed that the suppression of ASS1 could contribute to a decrease in arginine synthesis and may additionally induce abnormal phosphorylation of Akt, making cells more susceptible to genotoxic stress and leading to cell death; however, this study was performed in astrocytoma cells, non-small cell lung cancer, lung adenocarcinoma cells, and colorectal adenocarcinoma cells [25].