Finally, as metabolic diseases (like obesity and diabetes), physical activity, diet interventions and other circulating factors as antioxidants, cytokines, IGF-I, IGFBP3 are associated with changes in microbiota composition and structure [64], we evaluated the impact of HIIT and/or T63 supplementation on the microbiota associated with the intestinal mucosa. The gene discussed is IGFBP3; the disease is obesity due to melanocortin 4 receptor deficiency.