Likewise, when we explored the effects of IL1β-silenced tumor cells on co-cultured NCFs, we could observe that cocultured fibroblasts lost the expression of inflammatory cytokines, as IL6, LIF or CCL2, while acquired markers of myofibroblasts (ACTA2, PDPN, MYH11, or CNN1; Figure 4H). Here, ACTA2 is linked to neoplasm.