SDHB and ischemia: To find out which active site of Complex II (SDH) is involved in ROS synthesis after ischemia/reperfusion, we inhibited the FAD binding site of Complex II (F site) with malonate, and found that the ability of this site to participate in ROS generation was inhibited after 30 and 40 min of ischemia with 30 min reperfusion; however, malonate had no effect on ROS after prolonged ischemia/reperfusion (Figure 10a and Figure 11a).