Cyclin dependent kinase 5 (CDK5) and Twist Family BHLH Transcription Factor 1 (TWIST1) have been reported to increase metastasis through regulating cell cycle and EMT and they were found to be upregulated in osteosarcoma tumors with low levels of 14q32 miRNAs [47,174,175]. The gene discussed is CDK5; the disease is osteosarcoma.