The in vivo anti-inflammatory activity of the E. oleracea seed extract was also observed in an experiment with C57BL/6 mice, in which açaí seed extract supplementation protected from obesity-associated hepatic steatosis and fibrosis, reducing oxidative stress, NF-κB expression and pro-inflammatory markers IL-6 and TNF-α [52]. The gene discussed is TNF; the disease is fatty liver disease.