CDH1 and CDH1-related diffuse gastric and lobular breast cancer syndrome: The methylation analysis performed on the germline DNA derived from blood of 6 healthy controls and of 20 HDGC CDH1-negative patients (15 with DGC and 5 with LBC) revealed a strongly conserved methylation pattern along promoter and enhancer sequences, with absence of methylation in the promoter and almost full methylation in enhancer B in all patients and controls (Figure 3 and Supplementary Figures S1 and S2).