In order to verify if the peritumoral immune contexture was more cytotoxic or more immune-suppressive and thus evaluate the balance between cytotoxic and immune suppressive activity in the melanoma microenvironment, we simultaneously quantified the number of CD3, GRZB and FOXP3-stained cells by a multiplex staining approach on all analyzed cases (Figure 2). This evidence concerns the gene FOXP3 and melanoma.