Subsequently, we conducted a study of 200 FFPE samples from metastatic melanoma patients treated with ipilimumab in which different immune populations were assessed using a digital image analysis application to characterize the immune infiltrate expression of CD3, CD8, CD20, FOXP3, CD163 and PD-L1, in order to investigate the predictive power of the immunoscore. The gene discussed is CD163; the disease is metastatic melanoma.