TRIP13 and glioblastoma: In order to analyze whether TRIP13 methylation levels could also affect these gene expression patterns, we initially used the TCGA LGG and GBM methylation dataset to identify the 118 genes in LGG and 14 genes in GBM (a portion of 121 gene lists), and generated a volcano plot to visualize the distribution of differentially expressed genes (DEGs), including significantly upregulated and downregulated genes associated with TRIP13 methylation (Figure 8D,E).