ALK and neoplasm: Figure 1 shows the representative FISH, IHC, and SRM results. The results of ALK FISH, ALK IHC, and SRM were compared with respect to the fixation status. A positive ALK FISH (>20% tumor cells), positive IHC, and positive SRM were significantly associated with direct fixation (p < 0.05) (Table 3). The ALK protein was not detected using IHC in specimens with delayed fixation, while SRM could detect the ALK protein in these cases (case numbers 12 and 14, Table S1).