Finally, quantitative RT-PCR results showed that the expression of the EMT transcription factor (EMT-TF) Twist decreased in the three BMAL1-KD CRC cell lines compared to their respective control, whereas no significant changes in the mRNA levels of the two other key EMT-TFs, Slug and Snail, were observed (Figure 3D). This evidence concerns the gene SNAI1 and colorectal carcinoma.