Here, we evaluated in vitro how knockdown BMAL1 (BMAL1-KD) directly affects EMT as well as migration/invasion and the drug-resistance capacities of two primary human CRC cell lines (i.e., HCT116 and SW480) and a metastatic CRC cell line (i.e., SW620). The gene discussed is BMAL1; the disease is colorectal carcinoma.