Our study revealed that BMAL1-KD reinforced epithelial properties of CRC cells with elevated plasma membrane co-localization of E-cadherin and β-catenin as well as a decreased β-catenin nuclear location, suggesting a decreased Wnt/β-catenin pathway activity in BMAL1-KD CRC cell lines. The gene discussed is CDH1; the disease is colorectal carcinoma.