In 2009, the ex-vivo co-electroporation of human DCs with a combination of immunostimulatory mRNAs encoding CD40 ligand, CD70 and a constitutively active TLR- 4 (TriMix DC) plus mRNAs encoding melanoma antigens MAGE-A3, MAGE-C2, gp100 and tyrosinase (TriMixDC-Mel), induced DC maturation and generated antigen-specific T-cells for the tumour associated antigens tested ex vivo [187]. Here, TYR is linked to melanoma.