To assess the modulatory impact of SAA1 binding on the processes of entry, intracellular growth, and survival of Mtb in human macrophages, tubercle bacilli were subjected to interaction with the acute phase protein at the physiological concentration (3 μg/mL) and 5-fold higher concentration (15 μg/mL) prior to the in vitro infection of MDM cultures at an MOI of 10:1. Here, SAA1 is linked to infection.