D1(Mg + Ca + K) can both effectively enhance antioxidative enzyme (SOD, CAT, GPx, and GRd) activities and increase fatty acid β-oxidation–related protein (AMPK and PPAR-α) expression levels as well as reduce fatty acid synthesis—related protein (FAS) expression levels, thus increasing fatty acid oxidation and decreasing lipid synthesis and then achieving the optimal effect in mitigating NAFLD. The gene discussed is PPARA; the disease is metabolic dysfunction-associated steatotic liver disease.