Four of them, Mannan-binding lectin serine protease 2 (MASP2) Calbindin (CALB1), S100A8 and S100A9, were selected as potential biomarkers of early DKD based on bioinformatic analysis, although no validation of the free or EV-encapsulated proteins was performed in either urine or blood (Table 2) [77]. This evidence concerns the gene CALB1 and diabetic kidney disease.