BAK1 and melanoma: In addition, we demonstrated that BA elicited a dose-dependent cytotoxic effect characterized by apoptotic features: morphological alterations (nuclear fragmentation, apoptotic bodies—Figure 4 and Figure 5), a decrease in mitochondrial membrane potential (Figure 6), up-regulation of pro-apoptotic markers mRNA expression (Bax, Bad and Bak) (Figure 7) and changes in mitochondria morphology and localization in the A375 melanoma cell line (Figure 8).