Our data are in line with a recent study demonstrating that citrate synthase activity, glycolysis rate and carbon flux through the TCA cycle were inhibited in BA-loaded nanoliposomes-treated colorectal cancer cells; the results were confirmed by the glycolysis stress test in which ECAR values revealed that both the basal glycolysis and the maximum glycolytic capacity were reduced by BA-loaded nanoliposomes [34]. This evidence concerns the gene CS and colorectal cancer.