To test the silencing activity of nanostructures 1/2 towards the endogenous mRNA of EGFR protein, we used human squamous cell carcinoma cells (A431) expressing higher levels of EGF receptor compared to HeLa (human cervical carcinoma) and MCF-7 (human breast cancer) cell lines [35]. The gene discussed is EGFR; the disease is squamous cell carcinoma.