To enhance the immunogenicity of the pertussis antigens, we first fused CTB or StxB to the N-terminals of Fim2, PtxS1, or FHA1877–2250 by constructing pET-CTB-Fim2 and pET-StxB-Fim2-SUMO, pET-CTB-PtxS1-SUMO, pET-StxB-PtxS1-SUMO, and pET-CTB-FHA1877–2250 and pET-StxB-FHA1877–2250 expression vectors, respectively. Here, CSF1R is linked to pertussis.