CSF1R and pertussis: After confirming that the endotoxin content of all vaccines including Fim2, StxB-Fim2, CTB-Fim2, PtxS1, StxB-PtxS1, CTB-PtxS1, FHA1877–2250, StxB-FHA1877–2250, and CTB-FHA1877–2250 were at a low level (Figure S2), each of the six purified CTB- or StxB-based fusion antigens and three unfused antigens (2 μg of pertussis antigen/mouse), along with 100 μg aluminum hydroxide adjuvant, were used to immunize five-week-old female BALB/c mice subcutaneously on Days 0, 14, and 28.