In view of the results of the present study observed elevated DRM ceramide level, lipid raft-associated p110α-free p85α–unphosphorylated PTEN complex formation, reduced the levels of DRM sphingomyelin and cholesterol, declined phosphorylation of Akt (Ser 473) and PTEN (Ser 380/Thr 382/Ser 385), aborted Rac activation, enhanced induction of p-CDK1 (Thr 161), p-BCL-xL (Ser 62), p-BCL-2 (Thr 69/Ser 87), cytosolic Ca++ and ROS, decreased ATP level, and cell death after treatment of DOPC, an inducer of ASM activity in NPC cells. This evidence concerns the gene SMPD1 and nasopharyngeal carcinoma.