Out of 50 analyzed astrocytoma samples of different grades, 41 (82%) had an unmethylated GSK3β gene promoter, while methylation of the promoter region was detected in nine samples (18%), including three AII (30%), three AIII (27%), and three GBM (10.34%) (Figure 3A). The gene discussed is GSK3B; the disease is astrocytoma (excluding glioblastoma).