Comparing the efficacy of the DNA vaccines in the present study (pOVAv and pOVAy) with those investigated in our previous study (pUbV-OVAy) [22], two-time pOVAv and pUbV-OVAy vaccines were found to have similar effects in terms of anti-tumor immunity and CD8+ T cell induction compared to the pOVAy vaccine, which was less effective (Figure 3). This evidence concerns the gene CD8A and neoplasm.