We identified EE-84 as having the most potent antiproliferative effect in several chronic myeloid leukemia cell lines (K562, KBM5, MEG01, K562IR, and KBM5IR) as shown by trypan blue exclusion tests, CFAs, Hoechst/PI, and Annexin V/PI staining assays. This evidence concerns the gene ANXA5 and chronic myelogenous leukemia, BCR-ABL1 positive.