The modulation on transcription factors involved in the stemness phenotype was further elucidated in the CSC-enriched lung cancer cells cultured with jorunnamycin A. The mRNA level of Nanog, Oct-4, and Sox2 determined by reverse transcription quantitative real-time PCR (RT-qPCR) is indicated in Figure 3A. Jorunnamycin A at 0.1–0.5 μM dramatically downregulated mRNA level of Nanog, Oct-4, and Sox2 in CSC H460 spheroids promptly at 24 h of treatment. Here, SOX2 is linked to lung cancer.