We performed immunohistochemical analyses on post-treatment brains of each experimental group and investigated infiltrations of tumor tissues by T cells and macrophages/microglia using CD3, CD4, CD8 (as illustrated in Figure 2B), CD11b, CD163, C204 (as illustrated in Figure 2C), and PD1 and PD-L1 (as illustrated in Supplementary Figure S4). Here, CD163 is linked to neoplasm.