To deepen our knowledge of RUNX1 implications in the NAFLD pathogenesis, we endeavored to analyze correlations between the RUNX1 relative expression in liver and different parameters related to NAFLD, such as weight, BMI, glucose, insulin, liver transaminases, different adipocytokines, some lipid metabolism–related genes (SREBP1c, FAS, LXRα, FXR, PPARα and PPARγ), and TLRs (TLR2, TLR4, TLR5 and TLR9). The gene discussed is TLR4; the disease is metabolic dysfunction-associated steatotic liver disease.