Upon isolation, CTCs from patients with Alveolar Soft Part Sarcoma have been efficiently identified using a multiplex immune-phenotyping assay to CTC biomarkers vimentin (VIM), cytokeratin (CK), and β-catenin (β-cat), while CD45+ cells have been excluded, to increase the confidence of cell identification and potentially include CTCs from different sarcoma [221]. Here, VIM is linked to sarcoma.