To evaluate the effect of Gal-1 inhibition by OTX008 on cell proliferation, six thyroid cancer cell lines (two papillary lines—TPC1, BCPAP; two follicular lines—FTC133, TT2609C02; and two anaplastic lines—8505c, CAL62) were cultured with a large range of OTX008 concentrations for 72 h and stained with crystal violet to evaluate cell proliferation. This evidence concerns the gene LGALS1 and thyroid gland carcinoma.