The inhibitor also reduced pS2 levels and a significant decrease in global mRNA expression, including 2-4 log2 fold reduction in CRC (Core Regulatory Circuitry) genes like MYC, MYB and RUNX1. ChIP-seq analysis following NVP-2 treatment revealed increased levels of promoter-proximal paused RNAP II at the TSS (Transcription Start Site), with corresponding decrease in RNAP II level all-over the gene body, including at the MYC, MYB and RUNX1 loci. This evidence concerns the gene MYC and colorectal carcinoma.