LCN2 and malignant glioma: Western blot and RT-qPCR analysis of human malignant glioma cell lines (GBM8901, U-87MG, U-251, GBM8401 and M059K) showed that GBM8901, U-87MG, U-251 and M059K cell lines had lower protein and mRNA expression of LCN2 than the GBM8401 cells (Figure 1C,D).