Analysis of the frequencies of T. cruzi-specific T cells with every possible combination of the five T-cell functions using the Boolean gating tool showed that in subjects without signs of heart disease (i.e., the G0 group), IL-27 mainly increased the frequencies of monofunctional IFN-γ-, IL-2-, and CD154-producing CD4+ T cells, whereas IL-7 increased the frequencies of monofunctional IFN-γ+ and MIP-1β+ CD4+ T cells (Figs 5C and S2). This evidence concerns the gene CD4 and heart disorder.