We had previously demonstrated that a Salmonella strain expressing an SseJ-hemagglutinin (HA) fusion tag was competent for SPI-2-dependent SseJ secretion (Chakraborty et al., 2015; Gao et al., 2018); we used this strain to infect HeLa cells, and fixed and immunostained after 16 hr post-infection (Figure 4). The gene discussed is CHGA; the disease is infection.