In this study, we found, for the first time, that PP7 possessed potent anti-angiogenic and anti-metastatic activities against HCC in transgenic Tg (fli1:EGFP) zebrafish model, zebrafish tumor xenograft model and lung metastasis mouse model, which were attributable, at least partially, to the downregulation of the NF-κB/MMP-9/VEGF pathway. This evidence concerns the gene VEGFA and neoplasm.