NOG and malignant colon neoplasm: To investigate the effect of VP on human colon cancers, we successfully modeled organoids from patients with colon cancer in a medium supplemented with the growth factors noggin, epidermal growth factor (EGF), human fibroblast growth factor 10 (FGF-10), HEPES, GlutaMAX, Y-27632, and A83-01 (Figure 5A).