Takemae et al. (2013) reported that TUBB2C and TUBB5 interact with TgRON4. In our study, TUBB2C and TUBB5 were not identified as DEPs in the HT vs. H group, but other alpha- and beta-tubulin proteins were detected, including TUBA1C (0.625 fold), TUBA4A (0.595 fold), TUBB2A (0.801 fold), TUBB3 (0.688 fold), TUBB4B (0.646 fold), and TUBB6 (0.753 fold) (Supplementary Table S1A), indicating that infection with T. gondii causes the remodeling of the host cytoskeleton. This evidence concerns the gene TUBB3 and infection.