So, we used X-tile to determine the optimal prognostic cut-off value of each tumor marker, and as shown in Table 2, the optimal cut-off values of CEA, CA19-9, CA125, CA242, CA211, and CA15-3 were 2.15 ng/mL, 17.30 U/mL, 9.05 U/mL, 8.85 U/mL, 1.15 ng/mL and 16.00 U/mL, respectively. This evidence concerns the gene CEACAM5 and neoplasm.